geo.be Metadata Catalog
Protist community DNA metabarcoding
UNDER EMBARGO - This dataset is part of BE/2023 sampling campagn in SW Greenland fjords (Igaliku and Tunulliarfik). Samples for DNA extraction were collected along fjord transects at several depths of the water column. The focus of the study was to determine the taxonomical composotion of protist community in two Arctic fjords.
Simple
- Datum (Erstellung)
- 2026-06-16
- Datum (Überarbeitung)
- 2026-06-16
- Datum (Publikation)
- 2026-06-16
- Datum (Erstellung)
- 2016-05-19
- Identifikator
-
http://metadata.naturalsciences.be
/
bmdc.be:dataset:3144
- Datum
- Weitere Informationen
- This dataset is composed of the following sources: Marta Mikhno, Koen Sabbe (n.d.). Protist community DNA metabarcoding.
Verwalter
Königliches Belgisches Institut für Naturwissenschaften (KBIN), Operationelle Direktion Natürliche Umwelt (OD Natur), Belgian Marine Data Centre (BMDC)
https://www.bmdc.be
https://www.bmdc.be
Autor
Laboratorium voor Protistologie en Aquatische Ecologie, Departement Biologie (UGent)
Krijgslaan 281 S8, 9000 Gent, Belgium
,
Herausgeber
Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur)
https://www.naturalsciences.be
https://www.naturalsciences.be
Eigentümer / Datenherr
Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur)
https://www.naturalsciences.be
https://www.naturalsciences.be
- Keywords Schlüsselwörter
-
- Reporting INSPIRE
- Bundesregierung
- Nutzungseinschränkungen
- Benutzerdeifinierte Einschränkungen
- Andere Einschränkungen
- No conditions apply to access and use
- Andere Einschränkungen
- https://creativecommons.org/licenses/by/4.0/
- Zugriffseinschränkungen
- Benutzerdeifinierte Einschränkungen
- Andere Einschränkungen
- Öffentliche Zugang nicht beschränkt.
- Anwendungseinschränkungen
- Es gelten keine Bedingungen zum Nutzung.
- Sicherheitseinstufung
- Unbeschränkt
- Räumliche Darstellungsart
- Text, Tabelle
- Sprache
- English
- Thematik
-
- Meere
N
S
E
W
))
- gml32:beginPosition
- 2023-07-17
- gml32:endPosition
- 2023-07-31
- Identifikator des Referenzsystems
- http://www.opengis.net/def/crs/EPSG/0/4258
- Online
- Digital Object Identifier (DOI) ( DOI )
- Bezugsebene
- Datenbestand
Konformitätsergebnis
- Datum (Publikation)
- 2010-12-08
- Erklärung
- Siehe die angegebene Spezifikation
- Bestanden
- Ja
- Erläuterung
- Seawater samples (500 mL) were prefiltered (100 µm) and filtered onto 0.22 µm membrane filters, then stored at −80 °C. DNA was extracted using the DNeasy PowerLyzer Microbial Kit (Qiagen) following the manufacturer’s protocol, including negative extraction controls. The V4 region of the 18S rRNA gene was amplified using the TAReuk454FWD1 and TAReukREV3 primers (Stoeck et al., 2010). Amplicon libraries were sequenced on an Illumina MiSeq platform (2 × 300 bp). Sequence data were processed using the DADA2 pipeline (v.1.14.1) in R. Primers were removed, and reads were quality filtered, trimmed, and truncated (forward reads at 280 bp). Amplicon Sequence Variants (ASVs) were inferred after dereplication and error learning, and chimeras were removed. Due to low quality of reverse reads, only forward reads were retained for downstream analyses. Taxonomic assignment was performed against the PR2 database (v.5.0.0) with a minimum bootstrap confidence of 98%. Subsequent processing (phyloseq) included removal of non-eukaryotic sequences, Metazoa, unassigned ASVs, and potential contaminants (>1% in negative controls).
- Metadatensatzidentifikator
- bmdc.be:dataset:3144 XML
- Sprache
- English
- Zeichensatz
- Utf8
- Hierarchieebene
- Datenbestand
- Datum
- 2026-06-16T13:48:23.520Z
- Bezeichnung des Metadatenstandards
- Geographic information -- Metadata
- Version des Metadatenstandards
- ISO 19115:2003/19139
