bmdc.be:dataset:3144 English UTF-8 Dataset Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC) Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC) Institut royal des Sciences naturelles de Belgique (IRSNB), Direction opérationnelle Milieux naturels (DO Nature), Belgian Marine Data Centre (BMDC) Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN), Operationele Directie Natuurlijk Milieu (OD Natuur), Belgian Marine Data Centre (BMDC) Königliches Belgisches Institut für Naturwissenschaften (KBIN), Operationelle Direktion Natürliche Umwelt (OD Natur), Belgian Marine Data Centre (BMDC) Vautierstraat 29 Vautierstraat 29 Vautierstraat 29 Rue Vautier 29 Vautierstraat 29 Brussel Brussels Brussel Bruxelles Brüssel 1000 Belgium Belgium Belgique België Belgien bmdc@naturalsciences.be https://www.bmdc.be http Point of contact 2026-06-16T13:48:23.520Z Geographic information -- Metadata ISO 19115:2003/19139 http://www.opengis.net/def/crs/EPSG/0/4258 Protist community DNA metabarcoding 2026-06-16 Creation 2026-06-16 Revision 2026-06-16 Publication Digital Object Identifier 2016-05-19 https://doi.org/10.24417/bmdc.be:dataset:3144 Belgian Marine Data Centre bmdc.be:dataset:3144 http://metadata.naturalsciences.be This dataset is composed of the following sources: Marta Mikhno, Koen Sabbe (n.d.). Protist community DNA metabarcoding. UNDER EMBARGO - This dataset is part of BE/2023 sampling campagn in SW Greenland fjords (Igaliku and Tunulliarfik). Samples for DNA extraction were collected along fjord transects at several depths of the water column. The focus of the study was to determine the taxonomical composotion of protist community in two Arctic fjords. Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC) Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC) Institut royal des Sciences naturelles de Belgique (IRSNB), Direction opérationnelle Milieux naturels (DO Nature), Belgian Marine Data Centre (BMDC) Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN), Operationele Directie Natuurlijk Milieu (OD Natuur), Belgian Marine Data Centre (BMDC) Königliches Belgisches Institut für Naturwissenschaften (KBIN), Operationelle Direktion Natürliche Umwelt (OD Natur), Belgian Marine Data Centre (BMDC) Vautierstraat 29 Vautierstraat 29 Vautierstraat 29 Rue Vautier 29 Vautierstraat 29 Brussel Brussels Brussel Bruxelles Brüssel 1000 Belgium Belgium Belgique België Belgien bmdc@naturalsciences.be https://www.bmdc.be http Custodian Laboratorium voor Protistologie en Aquatische Ecologie, Departement Biologie (UGent) Krijgslaan 281 S8, 9000 Gent, Belgium Author Royal Belgian Institute of Natural Sciences (RBINS) Royal Belgian Institute of Natural Sciences (RBINS) Institut royal des Sciences naturelles de Belgique (IRSNB) Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN) Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur) Vautierstraat 29 Vautierstraat 29 Vautierstraat 29 Rue Vautier 29 Vautierstraat 29 Brussel Brussels Brussel Bruxelles Brüssel 1000 Belgium Belgium Belgique België Belgien info@naturalsciences.be https://www.naturalsciences.be http Publisher Royal Belgian Institute of Natural Sciences (RBINS) Royal Belgian Institute of Natural Sciences (RBINS) Institut royal des Sciences naturelles de Belgique (IRSNB) Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN) Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur) Vautierstraat 29 Vautierstraat 29 Vautierstraat 29 Rue Vautier 29 Vautierstraat 29 Brussel Brussels Brussel Bruxelles Brüssel 1000 Belgium Belgium Belgique België Belgien info@naturalsciences.be https://www.naturalsciences.be http Owner Reporting INSPIRE Reporting INSPIRE Reporting INSPIRE Reporting INSPIRE Reporting INSPIRE Federal government Federal government Federale Overheid Gouvernement Fédéral Bundesregierung Other restrictions No conditions apply to access and use https://creativecommons.org/licenses/by/4.0/ Other restrictions No limitations on public access. No limitations on public access. Geen beperkingen op openbare toegang. Pas de restrictions concernant l'accès public. Öffentliche Zugang nicht beschränkt. No conditions apply to use. No conditions apply to use. Geen voorwaarden in verband met gebruik. Aucune condition ne s'applique à l'utilisation. Es gelten keine Bedingungen zum Nutzung. Unclassified Text table English oceans true -46.58078 -45.36212 60.57022 61.15508 2023-07-17 2023-07-31 https://doi.org/10.24417/bmdc.be:dataset:3144 DOI Digital Object Identifier (DOI) Dataset COMMISSION REGULATION (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services COMMISSION REGULATION (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services Verordening (EU) n r. 1089/2010 van de Commissie van 23 november 2010 ter uitvoering van Richtlijn 2007/2/EG van het Europees Parlement en de Raad betreffende de interoperabiliteit van verzamelingen ruimtelijke gegevens en van diensten met betrekking tot ruimtelijke gegevens Règlement (UE) N° 1089/2010 de la Commission du 23 novembre 2010 portant modalités d'application de la directive 2007/2/CE du Parlement européen et du Conseil en ce qui concerne l'interopérabilité des séries et des services de données géographiques Verordnung (EG) Nr. 1089/2010 der Kommission vom 23. November 2010 zur Durchführung der Richtlinie 2007/2/EG des Europäischen Parlaments und des Rates hinsichtlich der Interoperabilität von Geodatensätzen und -diensten 2010-12-08 Publication See the referenced specification See the referenced specification Zie de gerefereerde specificatie Voir la spécification référencée Siehe die angegebene Spezifikation true Seawater samples (500 mL) were prefiltered (100 µm) and filtered onto 0.22 µm membrane filters, then stored at −80 °C. DNA was extracted using the DNeasy PowerLyzer Microbial Kit (Qiagen) following the manufacturer’s protocol, including negative extraction controls. The V4 region of the 18S rRNA gene was amplified using the TAReuk454FWD1 and TAReukREV3 primers (Stoeck et al., 2010). Amplicon libraries were sequenced on an Illumina MiSeq platform (2 × 300 bp). Sequence data were processed using the DADA2 pipeline (v.1.14.1) in R. Primers were removed, and reads were quality filtered, trimmed, and truncated (forward reads at 280 bp). Amplicon Sequence Variants (ASVs) were inferred after dereplication and error learning, and chimeras were removed. Due to low quality of reverse reads, only forward reads were retained for downstream analyses. Taxonomic assignment was performed against the PR2 database (v.5.0.0) with a minimum bootstrap confidence of 98%. Subsequent processing (phyloseq) included removal of non-eukaryotic sequences, Metazoa, unassigned ASVs, and potential contaminants (>1% in negative controls).