geo.be Metadata Catalog
Protist community DNA metabarcoding
UNDER EMBARGO - This dataset is part of BE/2023 sampling campagn in SW Greenland fjords (Igaliku and Tunulliarfik). Samples for DNA extraction were collected along fjord transects at several depths of the water column. The focus of the study was to determine the taxonomical composotion of protist community in two Arctic fjords.
Simple
- Date (Création)
- 2026-06-16
- Date (Révision)
- 2026-06-16
- Date (Publication)
- 2026-06-16
- Date (Création)
- 2016-05-19
- Identificateur
-
http://metadata.naturalsciences.be
/
bmdc.be:dataset:3144
- Date
- Autres informations de référence
- This dataset is composed of the following sources: Marta Mikhno, Koen Sabbe (n.d.). Protist community DNA metabarcoding.
Gestionnaire
Institut royal des Sciences naturelles de Belgique (IRSNB), Direction opérationnelle Milieux naturels (DO Nature), Belgian Marine Data Centre (BMDC)
https://www.bmdc.be
https://www.bmdc.be
Auteur
Laboratorium voor Protistologie en Aquatische Ecologie, Departement Biologie (UGent)
Krijgslaan 281 S8, 9000 Gent, Belgium
,
Editeur (publication)
Institut royal des Sciences naturelles de Belgique (IRSNB)
https://www.naturalsciences.be
https://www.naturalsciences.be
Propriétaire
Institut royal des Sciences naturelles de Belgique (IRSNB)
https://www.naturalsciences.be
https://www.naturalsciences.be
- Mots clés
-
- Reporting INSPIRE
- Gouvernement Fédéral
- Contraintes d'utilisation
- Autres restrictions
- Autres contraintes
- No conditions apply to access and use
- Autres contraintes
- https://creativecommons.org/licenses/by/4.0/
- Contraintes d'accès
- Autres restrictions
- Autres contraintes
- Pas de restrictions concernant l'accès public.
- Limitation d'utilisation
- Aucune condition ne s'applique à l'utilisation.
- Restrictions de manipulation
- Non classifié
- Type de représentation spatiale
- Tabulaire
- Langue
- English
- Catégorie ISO
-
- Océans
N
S
E
W
))
- gml32:beginPosition
- 2023-07-17
- gml32:endPosition
- 2023-07-31
- Nom du système de référence
- http://www.opengis.net/def/crs/EPSG/0/4258
- Ressource en ligne
- Digital Object Identifier (DOI) ( DOI )
- Niveau
- Jeu de données
Résultat de conformité
- Date (Publication)
- 2010-12-08
- Explication
- Voir la spécification référencée
- Degré de conformité
- Oui
- Généralités sur la provenance
- Seawater samples (500 mL) were prefiltered (100 µm) and filtered onto 0.22 µm membrane filters, then stored at −80 °C. DNA was extracted using the DNeasy PowerLyzer Microbial Kit (Qiagen) following the manufacturer’s protocol, including negative extraction controls. The V4 region of the 18S rRNA gene was amplified using the TAReuk454FWD1 and TAReukREV3 primers (Stoeck et al., 2010). Amplicon libraries were sequenced on an Illumina MiSeq platform (2 × 300 bp). Sequence data were processed using the DADA2 pipeline (v.1.14.1) in R. Primers were removed, and reads were quality filtered, trimmed, and truncated (forward reads at 280 bp). Amplicon Sequence Variants (ASVs) were inferred after dereplication and error learning, and chimeras were removed. Due to low quality of reverse reads, only forward reads were retained for downstream analyses. Taxonomic assignment was performed against the PR2 database (v.5.0.0) with a minimum bootstrap confidence of 98%. Subsequent processing (phyloseq) included removal of non-eukaryotic sequences, Metazoa, unassigned ASVs, and potential contaminants (>1% in negative controls).
- Identifiant de la fiche
- bmdc.be:dataset:3144 XML
- Langue
- English
- Jeu de caractères
- Utf8
- Type de ressource
- Jeu de données
- Date des métadonnées
- 2026-06-16T13:48:23.520Z
- Nom du standard de métadonnées
- Geographic information -- Metadata
- Version du standard de métadonnées
- ISO 19115:2003/19139
