Protist community DNA metabarcoding

UNDER EMBARGO - This dataset is part of BE/2023 sampling campagn in SW Greenland fjords (Igaliku and Tunulliarfik). Samples for DNA extraction were collected along fjord transects at several depths of the water column. The focus of the study was to determine the taxonomical composotion of protist community in two Arctic fjords.

Datum (Erstellung) Date (Création) Datum (creatie) Date (Creation): 2026-06-16

Datum (Überarbeitung) Date (Révision) Datum (revisie) Date (Revision): 2026-06-16

Datum (Publikation) Date (Publication) Datum (publicatie) Date (Publication): 2026-06-16

Datum (Erstellung) Date (Création) Datum (creatie) Date (Creation): 2016-05-19

Unique resource identifier: https://doi.org/10.24417/bmdc.be:dataset:3144

Identifikator Identificateur Identifier Identifier

http://metadata.naturalsciences.be / bmdc.be:dataset:3144

Datum Date Datum Date

Other citation details: This dataset is composed of the following sources: Marta Mikhno, Koen Sabbe (n.d.). Protist community DNA metabarcoding.

Verwalter

Gestionnaire

beheerder

Custodian

Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC)

Institut royal des Sciences naturelles de Belgique (IRSNB), Direction opérationnelle Milieux naturels (DO Nature), Belgian Marine Data Centre (BMDC)

Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN), Operationele Directie Natuurlijk Milieu (OD Natuur), Belgian Marine Data Centre (BMDC)

Königliches Belgisches Institut für Naturwissenschaften (KBIN), Operationelle Direktion Natürliche Umwelt (OD Natur), Belgian Marine Data Centre (BMDC)

Vautierstraat 29

Rue Vautier 29

Vautierstraat 29

Brussels

Brussel

Bruxelles

Brüssel

, 1000 ,

https://www.bmdc.be

Autor

Auteur

auteur

Author

Laboratorium voor Protistologie en Aquatische Ecologie, Departement Biologie (UGent)

Krijgslaan 281 S8, 9000 Gent, Belgium ,

Herausgeber

Editeur (publication)

uitgever

Publisher

Royal Belgian Institute of Natural Sciences (RBINS)

Institut royal des Sciences naturelles de Belgique (IRSNB)

Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN)

Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur)

Vautierstraat 29

Rue Vautier 29

Vautierstraat 29

Brussels

Brussel

Bruxelles

Brüssel

, 1000 ,

https://www.naturalsciences.be

Eigentümer / Datenherr

Propriétaire

eigenaar

Owner

Royal Belgian Institute of Natural Sciences (RBINS)

Institut royal des Sciences naturelles de Belgique (IRSNB)

Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN)

Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur)

Vautierstraat 29

Rue Vautier 29

Vautierstraat 29

Brussels

Brussel

Bruxelles

Brüssel

, 1000 ,

https://www.naturalsciences.be

Keywords

Reporting INSPIRE

Reporting INSPIRE

Reporting INSPIRE

Reporting INSPIRE
Federal government

Federale Overheid

Gouvernement Fédéral

Bundesregierung

Nutzungseinschränkungen Contraintes d'utilisation (Juridische) gebruiksbeperking Use constraints: Benutzerdeifinierte Einschränkungen

Autres restrictions

anders

Other restrictions

Other constraints: No conditions apply to access and use

Other constraints: https://creativecommons.org/licenses/by/4.0/

Zugriffseinschränkungen Contraintes d'accès (Juridische) toegangsrestrictie Access constraints: Benutzerdeifinierte Einschränkungen

Autres restrictions

anders

Other restrictions

Other constraints Overige beperkingen Autres contraintes Andere Einschränkungen: No limitations on public access.

Geen beperkingen op openbare toegang.

Pas de restrictions concernant l'accès public.

Öffentliche Zugang nicht beschränkt.

Use limitation Gebruiksbeperkingen Limitation d'utilisation Anwendungseinschränkungen: No conditions apply to use.

Geen voorwaarden in verband met gebruik.

Aucune condition ne s'applique à l'utilisation.

Es gelten keine Bedingungen zum Nutzung.

Sicherheitseinstufung Restrictions de manipulation Veiligheidsrestricties Classification: Unbeschränkt

Non classifié

vrij toegankelijk

Unclassified

Räumliche Darstellungsart Type de représentation spatiale Ruimtelijk schema Spatial representation type: Text, Tabelle

Tabulaire

tekstTabel

Text, table

Sprache Langue Taal Metadata language: English

Thematik Catégorie ISO Onderwerp Topic category

Meere

Océans

oceanen

Oceans

gml32:beginPosition: 2023-07-17

gml32:endPosition: 2023-07-31

Identifikator des Referenzsystems Nom du système de référence Referentiesysteem identifier Reference system identifier: http://www.opengis.net/def/crs/EPSG/0/4258

Online Ressource en ligne OnLine bronnen OnLine resource: Digital Object Identifier (DOI) ( DOI )

Bezugsebene Niveau Niveau kwaliteitsbeschrijving Hierarchy level: Datenbestand

Jeu de données

dataset

Dataset

Konformitätsergebnis

Résultat de conformité

Resultaat conformiteit met de specificatie

Conformance result

Datum (Publikation) Date (Publication) Datum (publicatie) Date (Publication): 2010-12-08

Explanation Uitleg Explication Erklärung: See the referenced specification

Zie de gerefereerde specificatie

Voir la spécification référencée

Siehe die angegebene Spezifikation

Bestanden Degré de conformité Indicatie van conformiteit met de specificatie Pass: Yes

Statement: Seawater samples (500 mL) were prefiltered (100 µm) and filtered onto 0.22 µm membrane filters, then stored at −80 °C. DNA was extracted using the DNeasy PowerLyzer Microbial Kit (Qiagen) following the manufacturer’s protocol, including negative extraction controls. The V4 region of the 18S rRNA gene was amplified using the TAReuk454FWD1 and TAReukREV3 primers (Stoeck et al., 2010). Amplicon libraries were sequenced on an Illumina MiSeq platform (2 × 300 bp). Sequence data were processed using the DADA2 pipeline (v.1.14.1) in R. Primers were removed, and reads were quality filtered, trimmed, and truncated (forward reads at 280 bp). Amplicon Sequence Variants (ASVs) were inferred after dereplication and error learning, and chimeras were removed. Due to low quality of reverse reads, only forward reads were retained for downstream analyses. Taxonomic assignment was performed against the PR2 database (v.5.0.0) with a minimum bootstrap confidence of 98%. Subsequent processing (phyloseq) included removal of non-eukaryotic sequences, Metazoa, unassigned ASVs, and potential contaminants (>1% in negative controls).