geo.be Metadata Catalog
[UNDER EMBARGO] Microbial growth and micro, mesozooplankton grazing
UNDER EMBARGO - This dataset originates from the BE/2023 sampling campaign conducted in southwest Greenland fjords (Igaliku and Tunulliarfik) and quantifies grazing impacts by micro- and mesozooplankton on phytoplankton and heterotrophic microbial communities (including bacteria) in two fjord systems characterized by contrasting glacial regimes. Grazing and microbial growth rates were estimated using two-point dilution experiments (two-point dilution experiments), alongside experiments assessing mesozooplankton and copepod grazing on both phytoplankton and microzooplankton. Community responses were resolved using imaging flow cytometry, enabling the identification of plankton functional groups (autotrophic, mixotrophic, and heterotrophic) and size classes. The dataset also includes measurements of chlorophyll a variability determined by high-performance liquid chromatography. Overall, the dataset supports analyses of trophic interactions and grazing dynamics across the microbial food web under differing glacier-influenced environmental conditions.
Simple
- Datumsangaben (Erstellung)
- 2026-06-16
- Datumsangaben (Überarbeitung)
- 2026-10-21
- Datumsangaben (Publikation)
- 2026-10-21
- Titel
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Digital Object Identifier
- Datumsangaben (Erstellung)
- 2016-05-19
- Identifikator
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http://metadata.naturalsciences.be
/
bmdc.be:dataset:3141
Belgian Marine Data Centre
- Kontakt für die Ressource
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Organisation Person E-Mail Funktion Königliches Belgisches Institut für Naturwissenschaften (KBIN), Operationelle Direktion Natürliche Umwelt (OD Natur), Belgian Marine Data Centre (BMDC)
Verwalter Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur)
Eigentümer / Datenherr Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur)
Herausgeber
- Keywords
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flow cytometry
- Greenland
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fjord
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grazing
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protist
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Reporting INSPIRE
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Bundesregierung
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- Nutzungseinschränkungen
- Benutzerdefinierte Einschränkungen
- Andere Einschränkungen
- No conditions apply to access and use
- Andere Einschränkungen
- https://creativecommons.org/licenses/by/4.0/
- Zugriffseinschränkungen
- Benutzerdefinierte Einschränkungen
- Andere Einschränkungen
- Öffentliche Zugang nicht beschränkt.
- Anwendungseinschränkungen
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Es gelten keine Bedingungen zum Nutzung.
- Sicherheitseinstufung
- Unbeschränkt
- Räumliche Darstellungsart
- Text, Tabelle
- Sprache
- English
- ISO-Thematik
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- Meere
))
- gml32:beginPosition
- 2023-07-18
- gml32:endPosition
- 2023-07-31
- Identifikator des Referenzsystems
- http://www.opengis.net/def/crs/EPSG/0/4258
Digitale Transferoptionen
- Online
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Protokoll URL Bezeichnung DOI
https://doi.org/10.24417/bmdc.be:dataset:3141 Digital Object Identifier (DOI)
- Bezugsebene
- Datenbestand
Konformitätsergebnis
- Datumsangaben (Publikation)
- 2010-12-08
- Erklärung
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Siehe die angegebene Spezifikation
- Bestanden
- Ja
- Erläuterung
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Because the upper size detection limit of this instrument (iFCM) is 100 µm, 14 mL of each seawater sample was pre-filtered through a 100 µm mesh to remove larger particles. The filtrate was subsequently fixed with glutaraldehyde (final concentration 1.0%) and stored at 4 °C. Samples were allowed to sediment for at least 48 h, after which the supernatant was carefully removed to obtain a final 7× concentration. This concentration step was necessary to compensate for the relatively low abundance of larger cells within the small analytical volume processed by the instrument.Prior to acquisition, 0.5 mL of each sample was stained with 1% (v/v) SYBR Green I (100× working solution in DMSO) and incubated in the dark at 37 °C for 20 min. Microscopic images (40× magnification; pixel size 0.5 × 0.5 µm) and flow cytometry signals, including brightfield images, fluorescence at 488 nm (SYBR Green–stained nucleic acids), and fluorescence at 642 nm (chlorophyll autofluorescence), were recorded for further analysis.Using IDEAS® software (v. 6.2), plankton populations were identified and quantified based on their optical and fluorescence characteristics. For chlorophyll a analysis, seawater volumes ranging from 400 mL to 600 mL were filtered onto 25-mm diameter Whatman GF/F filters and immediately stored at -80°C until further analysis. Pigments were extracted using 90% acetone and analysed by High-Performance Liquid Chromatography (HPLC) following the method of Van Heukelem and Thomas (2001). Calibration was performed using pigment standards from DHI Water and Environment (Hørsholm, Denmark). In the dataset is indicated the final consentration (μg/L) of each detected photosyntetic pigment.
Metadaten
- Metadatensatzidentifikator
- bmdc.be:dataset:3141 XML
- Sprache
- English
- Zeichensatz
- Utf8
- Hierarchieebene
- Datenbestand
- Datum
- 2026-10-21T16:13:47.657Z
- Bezeichnung des Metadatenstandards
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Geographic information -- Metadata
- Version des Metadatenstandards
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ISO 19115:2003/19139
- Metadatenkontakt
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Organisation Person E-Mail Funktion Königliches Belgisches Institut für Naturwissenschaften (KBIN), Operationelle Direktion Natürliche Umwelt (OD Natur), Belgian Marine Data Centre (BMDC)
Ansprechpartner
- Sprachraum
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Sprache Zeichensatz Deutsch Französisch Niederländisch Englisch
