bmdc.be:dataset:3141 English UTF-8 Dataset Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC) Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC) Institut royal des Sciences naturelles de Belgique (IRSNB), Direction opérationnelle Milieux naturels (DO Nature), Belgian Marine Data Centre (BMDC) Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN), Operationele Directie Natuurlijk Milieu (OD Natuur), Belgian Marine Data Centre (BMDC) Königliches Belgisches Institut für Naturwissenschaften (KBIN), Operationelle Direktion Natürliche Umwelt (OD Natur), Belgian Marine Data Centre (BMDC) Vautierstraat 29 Vautierstraat 29 Vautierstraat 29 Rue Vautier 29 Vautierstraat 29 Brussel Brussels Brussel Bruxelles Brüssel 1000 Belgium Belgium Belgique België Belgien bmdc@naturalsciences.be https://www.bmdc.be http Point of contact 2026-06-16T13:48:13.035Z Geographic information -- Metadata ISO 19115:2003/19139 http://www.opengis.net/def/crs/EPSG/0/4258 Microbial growth and micro, mesozooplankton grazing 2026-06-16 Creation 2026-06-16 Revision 2026-06-16 Publication Digital Object Identifier 2016-05-19 https://doi.org/10.24417/bmdc.be:dataset:3141 Belgian Marine Data Centre bmdc.be:dataset:3141 http://metadata.naturalsciences.be This dataset is composed of the following sources: Marta Mikhno, Koen Sabbe (n.d.). Microbial growth and micro, mesozooplankton grazing. UNDER EMBARGO - This dataset originates from the BE/2023 sampling campaign conducted in southwest Greenland fjords (Igaliku and Tunulliarfik) and quantifies grazing impacts by micro- and mesozooplankton on phytoplankton and heterotrophic microbial communities (including bacteria) in two fjord systems characterized by contrasting glacial regimes. Grazing and microbial growth rates were estimated using two-point dilution experiments (two-point dilution experiments), alongside experiments assessing mesozooplankton and copepod grazing on both phytoplankton and microzooplankton. Community responses were resolved using imaging flow cytometry, enabling the identification of plankton functional groups (autotrophic, mixotrophic, and heterotrophic) and size classes. The dataset also includes measurements of chlorophyll a variability determined by high-performance liquid chromatography. Overall, the dataset supports analyses of trophic interactions and grazing dynamics across the microbial food web under differing glacier-influenced environmental conditions. Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC) Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC) Institut royal des Sciences naturelles de Belgique (IRSNB), Direction opérationnelle Milieux naturels (DO Nature), Belgian Marine Data Centre (BMDC) Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN), Operationele Directie Natuurlijk Milieu (OD Natuur), Belgian Marine Data Centre (BMDC) Königliches Belgisches Institut für Naturwissenschaften (KBIN), Operationelle Direktion Natürliche Umwelt (OD Natur), Belgian Marine Data Centre (BMDC) Vautierstraat 29 Vautierstraat 29 Vautierstraat 29 Rue Vautier 29 Vautierstraat 29 Brussel Brussels Brussel Bruxelles Brüssel 1000 Belgium Belgium Belgique België Belgien bmdc@naturalsciences.be https://www.bmdc.be http Custodian Laboratorium voor Protistologie en Aquatische Ecologie, Departement Biologie (UGent) Krijgslaan 281 S8, 9000 Gent, Belgium Author Royal Belgian Institute of Natural Sciences (RBINS) Royal Belgian Institute of Natural Sciences (RBINS) Institut royal des Sciences naturelles de Belgique (IRSNB) Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN) Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur) Vautierstraat 29 Vautierstraat 29 Vautierstraat 29 Rue Vautier 29 Vautierstraat 29 Brussel Brussels Brussel Bruxelles Brüssel 1000 Belgium Belgium Belgique België Belgien info@naturalsciences.be https://www.naturalsciences.be http Publisher Royal Belgian Institute of Natural Sciences (RBINS) Royal Belgian Institute of Natural Sciences (RBINS) Institut royal des Sciences naturelles de Belgique (IRSNB) Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN) Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur) Vautierstraat 29 Vautierstraat 29 Vautierstraat 29 Rue Vautier 29 Vautierstraat 29 Brussel Brussels Brussel Bruxelles Brüssel 1000 Belgium Belgium Belgique België Belgien info@naturalsciences.be https://www.naturalsciences.be http Owner Reporting INSPIRE Reporting INSPIRE Reporting INSPIRE Reporting INSPIRE Reporting INSPIRE Federal government Federal government Federale Overheid Gouvernement Fédéral Bundesregierung Other restrictions No conditions apply to access and use https://creativecommons.org/licenses/by/4.0/ Other restrictions No limitations on public access. No limitations on public access. Geen beperkingen op openbare toegang. Pas de restrictions concernant l'accès public. Öffentliche Zugang nicht beschränkt. No conditions apply to use. No conditions apply to use. Geen voorwaarden in verband met gebruik. Aucune condition ne s'applique à l'utilisation. Es gelten keine Bedingungen zum Nutzung. Unclassified Text table English oceans true -46.58078 -45.36212 60.57022 61.15508 2023-07-18 2023-07-31 https://doi.org/10.24417/bmdc.be:dataset:3141 DOI Digital Object Identifier (DOI) Dataset COMMISSION REGULATION (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services COMMISSION REGULATION (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services Verordening (EU) n r. 1089/2010 van de Commissie van 23 november 2010 ter uitvoering van Richtlijn 2007/2/EG van het Europees Parlement en de Raad betreffende de interoperabiliteit van verzamelingen ruimtelijke gegevens en van diensten met betrekking tot ruimtelijke gegevens Règlement (UE) N° 1089/2010 de la Commission du 23 novembre 2010 portant modalités d'application de la directive 2007/2/CE du Parlement européen et du Conseil en ce qui concerne l'interopérabilité des séries et des services de données géographiques Verordnung (EG) Nr. 1089/2010 der Kommission vom 23. November 2010 zur Durchführung der Richtlinie 2007/2/EG des Europäischen Parlaments und des Rates hinsichtlich der Interoperabilität von Geodatensätzen und -diensten 2010-12-08 Publication See the referenced specification See the referenced specification Zie de gerefereerde specificatie Voir la spécification référencée Siehe die angegebene Spezifikation true Because the upper size detection limit of this instrument (iFCM) is 100 µm, 14 mL of each seawater sample was pre-filtered through a 100 µm mesh to remove larger particles. The filtrate was subsequently fixed with glutaraldehyde (final concentration 1.0%) and stored at 4 °C. Samples were allowed to sediment for at least 48 h, after which the supernatant was carefully removed to obtain a final 7× concentration. This concentration step was necessary to compensate for the relatively low abundance of larger cells within the small analytical volume processed by the instrument.Prior to acquisition, 0.5 mL of each sample was stained with 1% (v/v) SYBR Green I (100× working solution in DMSO) and incubated in the dark at 37 °C for 20 min. Microscopic images (40× magnification; pixel size 0.5 × 0.5 µm) and flow cytometry signals, including brightfield images, fluorescence at 488 nm (SYBR Green–stained nucleic acids), and fluorescence at 642 nm (chlorophyll autofluorescence), were recorded for further analysis.Using IDEAS® software (v. 6.2), plankton populations were identified and quantified based on their optical and fluorescence characteristics. For chlorophyll a analysis, seawater volumes ranging from 400 mL to 600 mL were filtered onto 25-mm diameter Whatman GF/F filters and immediately stored at -80°C until further analysis. Pigments were extracted using 90% acetone and analysed by High-Performance Liquid Chromatography (HPLC) following the method of Van Heukelem and Thomas (2001). Calibration was performed using pigment standards from DHI Water and Environment (Hørsholm, Denmark). In the dataset is indicated the final consentration (μg/L) of each detected photosyntetic pigment.