Date (Creation)

2026-06-16

Date (Revision)

2026-10-21

Date (Publication)

2026-10-21

Title

Digital Object Identifier

Date (Creation)

2016-05-19

Code

https://doi.org/10.24417/bmdc.be:dataset:3141

Citation identifier

http://metadata.naturalsciences.be / bmdc.be:dataset:3141

Belgian Marine Data Centre

Point of contact

Organisation name	Individual name	Electronic mail address	Role
Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC)		bmdc@naturalsciences.be	Custodian
Royal Belgian Institute of Natural Sciences (RBINS)		info@naturalsciences.be	Owner
Royal Belgian Institute of Natural Sciences (RBINS)		info@naturalsciences.be	Publisher

Keywords

• flow cytometry

• Greenland

• fjord

• grazing

• protist

• Reporting INSPIRE

• Federal government

GEMET - Concepts, version 4.2.3

• bacteria

GEMET - Concepts, version 4.2.1

• marine environment

Use constraints

Other restrictions

Other constraints

No conditions apply to access and use

Other constraints

https://creativecommons.org/licenses/by/4.0/

Access constraints

Other restrictions

Other constraints

No limitations on public access.

Use limitation

No conditions apply to use.

Classification

Unclassified

Spatial representation type

Text, table

Language

English

Topic category

• Oceans

gml32:beginPosition

2023-07-18

gml32:endPosition

2023-07-31

Reference system identifier

http://www.opengis.net/def/crs/EPSG/0/4258

Hierarchy level

Dataset

Statement

Because the upper size detection limit of this instrument (iFCM) is 100 µm, 14 mL of each seawater sample was pre-filtered through a 100 µm mesh to remove larger particles. The filtrate was subsequently fixed with glutaraldehyde (final concentration 1.0%) and stored at 4 °C. Samples were allowed to sediment for at least 48 h, after which the supernatant was carefully removed to obtain a final 7× concentration. This concentration step was necessary to compensate for the relatively low abundance of larger cells within the small analytical volume processed by the instrument.Prior to acquisition, 0.5 mL of each sample was stained with 1% (v/v) SYBR Green I (100× working solution in DMSO) and incubated in the dark at 37 °C for 20 min. Microscopic images (40× magnification; pixel size 0.5 × 0.5 µm) and flow cytometry signals, including brightfield images, fluorescence at 488 nm (SYBR Green–stained nucleic acids), and fluorescence at 642 nm (chlorophyll autofluorescence), were recorded for further analysis.Using IDEAS® software (v. 6.2), plankton populations were identified and quantified based on their optical and fluorescence characteristics. For chlorophyll a analysis, seawater volumes ranging from 400 mL to 600 mL were filtered onto 25-mm diameter Whatman GF/F filters and immediately stored at -80°C until further analysis. Pigments were extracted using 90% acetone and analysed by High-Performance Liquid Chromatography (HPLC) following the method of Van Heukelem and Thomas (2001). Calibration was performed using pigment standards from DHI Water and Environment (Hørsholm, Denmark). In the dataset is indicated the final consentration (μg/L) of each detected photosyntetic pigment.

Metadata

File identifier

bmdc.be:dataset:3141 XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Date stamp

2026-10-21T16:13:47.657Z

Metadata standard name

Geographic information -- Metadata

Metadata standard version

ISO 19115:2003/19139

Metadata author

Organisation name	Individual name	Electronic mail address	Role
Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC)		bmdc@naturalsciences.be	Point of contact

Other language

Language	Character encoding
German
French
Dutch; Flemish
English