Microbial growth and micro, mesozooplankton grazing

UNDER EMBARGO - This dataset originates from the BE/2023 sampling campaign conducted in southwest Greenland fjords (Igaliku and Tunulliarfik) and quantifies grazing impacts by micro- and mesozooplankton on phytoplankton and heterotrophic microbial communities (including bacteria) in two fjord systems characterized by contrasting glacial regimes. Grazing and microbial growth rates were estimated using two-point dilution experiments (two-point dilution experiments), alongside experiments assessing mesozooplankton and copepod grazing on both phytoplankton and microzooplankton. Community responses were resolved using imaging flow cytometry, enabling the identification of plankton functional groups (autotrophic, mixotrophic, and heterotrophic) and size classes. The dataset also includes measurements of chlorophyll a variability determined by high-performance liquid chromatography. Overall, the dataset supports analyses of trophic interactions and grazing dynamics across the microbial food web under differing glacier-influenced environmental conditions.

Datum (Erstellung) Date (Création) Datum (creatie) Date (Creation): 2026-06-16

Datum (Überarbeitung) Date (Révision) Datum (revisie) Date (Revision): 2026-06-16

Datum (Publikation) Date (Publication) Datum (publicatie) Date (Publication): 2026-06-16

Datum (Erstellung) Date (Création) Datum (creatie) Date (Creation): 2016-05-19

Unique resource identifier: https://doi.org/10.24417/bmdc.be:dataset:3141

Identifikator Identificateur Identifier Identifier

http://metadata.naturalsciences.be / bmdc.be:dataset:3141

Datum Date Datum Date

Other citation details: This dataset is composed of the following sources: Marta Mikhno, Koen Sabbe (n.d.). Microbial growth and micro, mesozooplankton grazing.

Verwalter

Gestionnaire

beheerder

Custodian

Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC)

Institut royal des Sciences naturelles de Belgique (IRSNB), Direction opérationnelle Milieux naturels (DO Nature), Belgian Marine Data Centre (BMDC)

Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN), Operationele Directie Natuurlijk Milieu (OD Natuur), Belgian Marine Data Centre (BMDC)

Königliches Belgisches Institut für Naturwissenschaften (KBIN), Operationelle Direktion Natürliche Umwelt (OD Natur), Belgian Marine Data Centre (BMDC)

Vautierstraat 29

Rue Vautier 29

Vautierstraat 29

Brussels

Brussel

Bruxelles

Brüssel

, 1000 ,

https://www.bmdc.be

Autor

Auteur

auteur

Author

Laboratorium voor Protistologie en Aquatische Ecologie, Departement Biologie (UGent)

Krijgslaan 281 S8, 9000 Gent, Belgium ,

Herausgeber

Editeur (publication)

uitgever

Publisher

Royal Belgian Institute of Natural Sciences (RBINS)

Institut royal des Sciences naturelles de Belgique (IRSNB)

Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN)

Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur)

Vautierstraat 29

Rue Vautier 29

Vautierstraat 29

Brussels

Brussel

Bruxelles

Brüssel

, 1000 ,

https://www.naturalsciences.be

Eigentümer / Datenherr

Propriétaire

eigenaar

Owner

Royal Belgian Institute of Natural Sciences (RBINS)

Institut royal des Sciences naturelles de Belgique (IRSNB)

Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN)

Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur)

Vautierstraat 29

Rue Vautier 29

Vautierstraat 29

Brussels

Brussel

Bruxelles

Brüssel

, 1000 ,

https://www.naturalsciences.be

Keywords

Reporting INSPIRE

Reporting INSPIRE

Reporting INSPIRE

Reporting INSPIRE
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Other constraints: https://creativecommons.org/licenses/by/4.0/

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vrij toegankelijk

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Räumliche Darstellungsart Type de représentation spatiale Ruimtelijk schema Spatial representation type: Text, Tabelle

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tekstTabel

Text, table

Sprache Langue Taal Metadata language: English

Thematik Catégorie ISO Onderwerp Topic category

Meere

Océans

oceanen

Oceans

gml32:beginPosition: 2023-07-18

gml32:endPosition: 2023-07-31

Identifikator des Referenzsystems Nom du système de référence Referentiesysteem identifier Reference system identifier: http://www.opengis.net/def/crs/EPSG/0/4258

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dataset

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Datum (Publikation) Date (Publication) Datum (publicatie) Date (Publication): 2010-12-08

Explanation Uitleg Explication Erklärung: See the referenced specification

Zie de gerefereerde specificatie

Voir la spécification référencée

Siehe die angegebene Spezifikation

Bestanden Degré de conformité Indicatie van conformiteit met de specificatie Pass: Yes

Statement: Because the upper size detection limit of this instrument (iFCM) is 100 µm, 14 mL of each seawater sample was pre-filtered through a 100 µm mesh to remove larger particles. The filtrate was subsequently fixed with glutaraldehyde (final concentration 1.0%) and stored at 4 °C. Samples were allowed to sediment for at least 48 h, after which the supernatant was carefully removed to obtain a final 7× concentration. This concentration step was necessary to compensate for the relatively low abundance of larger cells within the small analytical volume processed by the instrument.Prior to acquisition, 0.5 mL of each sample was stained with 1% (v/v) SYBR Green I (100× working solution in DMSO) and incubated in the dark at 37 °C for 20 min. Microscopic images (40× magnification; pixel size 0.5 × 0.5 µm) and flow cytometry signals, including brightfield images, fluorescence at 488 nm (SYBR Green–stained nucleic acids), and fluorescence at 642 nm (chlorophyll autofluorescence), were recorded for further analysis.Using IDEAS® software (v. 6.2), plankton populations were identified and quantified based on their optical and fluorescence characteristics. For chlorophyll a analysis, seawater volumes ranging from 400 mL to 600 mL were filtered onto 25-mm diameter Whatman GF/F filters and immediately stored at -80°C until further analysis. Pigments were extracted using 90% acetone and analysed by High-Performance Liquid Chromatography (HPLC) following the method of Van Heukelem and Thomas (2001). Calibration was performed using pigment standards from DHI Water and Environment (Hørsholm, Denmark). In the dataset is indicated the final consentration (μg/L) of each detected photosyntetic pigment.