Bacterial community DNA metabarcoding

UNDER EMBARGO - This dataset is part of BE/2023 sampling campagn in SW Greenland fjords (Igaliku and Tunulliarfik). Samples for DNA extraction were collected along fjord transects at several depths of the water column. The focus of the study was to determine the taxonomical composotion of bacterial community in two Arctic fjords.

Simple

Date (Creation)
2026-06-16
Date (Revision)
2026-06-16
Date (Publication)
2026-06-16
Date (Creation)
2016-05-19
Unique resource identifier
https://doi.org/10.24417/bmdc.be:dataset:3143
Identifier
http://metadata.naturalsciences.be / bmdc.be:dataset:3143

Date

Other citation details
This dataset is composed of the following sources: Marta Mikhno, Koen Sabbe (n.d.). Bacterial community DNA metabarcoding.
Custodian
  Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC)
Vautierstraat 29 , Brussels , 1000 , Belgium
https://www.bmdc.be
Author
  Laboratorium voor Protistologie en Aquatische Ecologie, Departement Biologie (UGent)
Krijgslaan 281 S8, 9000 Gent, Belgium ,
Publisher
  Royal Belgian Institute of Natural Sciences (RBINS)
Vautierstraat 29 , Brussels , 1000 , Belgium
https://www.naturalsciences.be
Owner
  Royal Belgian Institute of Natural Sciences (RBINS)
Vautierstraat 29 , Brussels , 1000 , Belgium
https://www.naturalsciences.be
Keywords
  • Reporting INSPIRE
  • Federal government
Use constraints
Other restrictions
Other constraints
No conditions apply to access and use
Other constraints
https://creativecommons.org/licenses/by/4.0/
Access constraints
Other restrictions
Other constraints
No limitations on public access.
Use limitation
No conditions apply to use.
Classification
Unclassified
Spatial representation type
Text, table
Metadata language
English
Topic category
  • Oceans
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gml32:beginPosition
2023-07-17
gml32:endPosition
2023-07-31
Reference system identifier
http://www.opengis.net/def/crs/EPSG/0/4258
OnLine resource
Digital Object Identifier (DOI) ( DOI )
Hierarchy level
Dataset

Conformance result

Date (Publication)
2010-12-08
Explanation
See the referenced specification
Pass
Yes
Statement
Seawater DNA was amplified targeting the full-length 16S rRNA gene using primers 27F and 1492R (Lane, 1991). PCR, library preparation, and barcoding followed Van Der Loos et al. (2021), including mock community and triplicate samples for quality control. Amplicons were sequenced on an Oxford Nanopore MinION platform (R9.4.1 flow cell, 72 h run). Raw reads were basecalled with Guppy, quality-checked (NanoPlot), and demultiplexed (qcat). Chimeras were removed (Yacrd), and reads were filtered by length (1000–2000 bp) and quality (Q >10) using NanoFilt. Sequences were clustered into Operational Taxonomic Units (OTUs) at 97% similarity, and taxonomy was assigned using Kraken2. Downstream processing (phyloseq) included removal of singletons, doubletons, non-bacterial sequences (chloroplasts and mitochondria), unclassified OTUs at phylum level, and potential contaminants (>1% relative abundance in negative controls).
File identifier
bmdc.be:dataset:3143 XML
Metadata language
English
Character set
UTF8
Hierarchy level
Dataset
Date stamp
2026-06-16T13:48:20.317Z
Metadata standard name
Geographic information -- Metadata
Metadata standard version
ISO 19115:2003/19139
Point of contact
  Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC)
Vautierstraat 29 , Brussels , 1000 , Belgium
https://www.bmdc.be
 
 

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