[UNDER EMBARGO] Pelagic community cell abundances and carbon biomass

UNDER EMBARGO - This dataset is part of BE/2023 sampling campagn in SW Greenland fjords (Igaliku and Tunulliarfik). Pelagic community was analysed using Imaging Flow Cytometry (iFCM) with an ImageStream®X Mk II. Cells were grouped into functional size classes—pico-, nano- and microplankton—according to measured cell length. Cells lacking chlorophyll autofluorescence were classified as heterotrophic or chemotrophic organisms, including heterotrophic picoplankton/bacteria (HP; ≤2 µm) and heterotrophic nanoplankton (HN; 2–20 µm). No larger heterotrophs (>20 µm) were visually detected. Autofluorescent cells were considered phototrophic, although this fraction may also include mixotrophic taxa, and comprised picophytoplankton (AP; ≤2 µm), nanophytoplankton (AN; 2–20 µm), and microphytoplankton (AMicro; 20–100 µm). To estimate the biovolume of each plankton class, the two-dimensional cell surface area measured by the IDEAS® imaging software was multiplied by the mean cell width, assuming that cell width approximates the third spatial dimension. Carbon biomass was subsequently derived from biovolume using established carbon–volume relationships. For the HP fraction, carbon content was estimated using the bacterial conversion proposed by Romanova and Sazhin (2010), where volume is expressed in µm³. Although the HP fraction may also include heterotrophic picoeukaryotes, and its biomass may therefore be partly underestimated, this conversion was applied because the fraction was assumed to be numerically dominated by bacteria. For the other protist groups, carbon biomass was derived following Menden-Deuer and Lessard (2000). Carbon values were converted from pg C cell⁻¹ to carbon biomass (µg C L⁻¹) based on cell abundance.

Simple

Datumsangaben (Erstellung)
Date (Création)
Datum (creatie)
Date (Creation)
2026-06-16
Datumsangaben (Überarbeitung)
Date (Révision)
Datum (revisie)
Date (Revision)
2026-10-21
Datumsangaben (Publikation)
Date (Publication)
Datum (publicatie)
Date (Publication)
2026-10-21
Title

Digital Object Identifier
Datumsangaben (Erstellung)
Date (Création)
Datum (creatie)
Date (Creation)
2016-05-19
Code
https://doi.org/10.24417/bmdc.be:dataset:3142
Identifikator
Identificateur
Identifier
Citation identifier
http://metadata.naturalsciences.be / bmdc.be:dataset:3142

Belgian Marine Data Centre

Point of contact
Organisation name Individual name Electronic mail address Role

Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC)

Institut royal des Sciences naturelles de Belgique (IRSNB), Direction opérationnelle Milieux naturels (DO Nature), Belgian Marine Data Centre (BMDC)

Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN), Operationele Directie Natuurlijk Milieu (OD Natuur), Belgian Marine Data Centre (BMDC)

Königliches Belgisches Institut für Naturwissenschaften (KBIN), Operationelle Direktion Natürliche Umwelt (OD Natur), Belgian Marine Data Centre (BMDC)

bmdc@naturalsciences.be

Verwalter
Gestionnaire
beheerder
Custodian

Royal Belgian Institute of Natural Sciences (RBINS)

Institut royal des Sciences naturelles de Belgique (IRSNB)

Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN)

Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur)

info@naturalsciences.be

Eigentümer / Datenherr
Propriétaire
eigenaar
Owner

Royal Belgian Institute of Natural Sciences (RBINS)

Institut royal des Sciences naturelles de Belgique (IRSNB)

Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN)

Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur)

info@naturalsciences.be

Herausgeber
Editeur (publication)
uitgever
Publisher
Keywords

  • flow cytometry
  • Greenland

  • fjord

  • Reporting INSPIRE

    Reporting INSPIRE

    Reporting INSPIRE

    Reporting INSPIRE

  • Federal government

    Federale Overheid

    Gouvernement Fédéral

    Bundesregierung
GEMET - Concepts, version 4.2.3
GEMET - Concepts, version 4.2.1
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https://creativecommons.org/licenses/by/4.0/
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vrij toegankelijk
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Text, Tabelle
Tabulaire
tekstTabel
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Sprache
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Language
English
ISO-Thematik
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  • Meere
    Océans
    oceanen
    Oceans
N
S
E
W
thumbnail




gml32:beginPosition
2023-07-17
gml32:endPosition
2023-07-31
Identifikator des Referenzsystems
Nom du système de référence
Referentiesysteem identifier
Reference system identifier
http://www.opengis.net/def/crs/EPSG/0/4258

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Options de transfert numérique
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DOI

 		https://doi.org/10.24417/bmdc.be:dataset:3142

Digital Object Identifier (DOI)
Bezugsebene
Niveau
Niveau kwaliteitsbeschrijving
Hierarchy level
Datenbestand
Jeu de données
dataset
Dataset

Konformitätsergebnis
Résultat de conformité
Resultaat conformiteit met de specificatie
Conformance result

Title
Titel
Spécification
Titel
COMMISSION REGULATION (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
Verordening (EU) n r. 1089/2010 van de Commissie van 23 november 2010 ter uitvoering van Richtlijn 2007/2/EG van het Europees Parlement en de Raad betreffende de interoperabiliteit van verzamelingen ruimtelijke gegevens en van diensten met betrekking tot ruimtelijke gegevens
Règlement (UE) N° 1089/2010 de la Commission du 23 novembre 2010 portant modalités d'application de la directive 2007/2/CE du Parlement européen et du Conseil en ce qui concerne l'interopérabilité des séries et des services de données géographiques
Verordnung (EG) Nr. 1089/2010 der Kommission vom 23. November 2010 zur Durchführung der Richtlinie 2007/2/EG des Europäischen Parlaments und des Rates hinsichtlich der Interoperabilität von Geodatensätzen und -diensten
Datumsangaben (Publikation)
Date (Publication)
Datum (publicatie)
Date (Publication)
2010-12-08
Explanation
Uitleg
Explication
Erklärung

See the referenced specification

Zie de gerefereerde specificatie

Voir la spécification référencée

Siehe die angegebene Spezifikation
Bestanden
Degré de conformité
Indicatie van conformiteit met de specificatie
Pass
Yes
Statement

Because the upper size detection limit of this instrument (iFCM) is 100 µm, 14 mL of each seawater sample was pre-filtered through a 100 µm mesh to remove larger particles. The filtrate was subsequently fixed with glutaraldehyde (final concentration 1.0%) and stored at 4 °C. Samples were allowed to sediment for at least 48 h, after which the supernatant was carefully removed to obtain a final 7× concentration. This concentration step was necessary to compensate for the relatively low abundance of larger cells within the small analytical volume processed by the instrument.Prior to acquisition, 0.5 mL of each sample was stained with 1% (v/v) SYBR Green I (100× working solution in DMSO) and incubated in the dark at 37 °C for 20 min. Microscopic images (40× magnification; pixel size 0.5 × 0.5 µm) and flow cytometry signals, including brightfield images, fluorescence at 488 nm (SYBR Green–stained nucleic acids), and fluorescence at 642 nm (chlorophyll autofluorescence), were recorded for further analysis.Using IDEAS® software (v. 6.2), plankton populations were identified and quantified based on their optical and fluorescence characteristics.

Metadata

Metadatensatzidentifikator
Identifiant de la fiche
Metadata ID
File identifier
bmdc.be:dataset:3142 XML
Sprache
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Taal
Metadata language
English
Zeichensatz
Jeu de caractères
Karakterset
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Utf8
Utf8
utf8
UTF8
Hierarchieebene
Type de ressource
Hierarchisch niveau
Hierarchy level
Datenbestand
Jeu de données
dataset
Dataset
Datum
Date des métadonnées
Metadata datum
Date stamp
2026-10-21T16:13:47.994Z
Metadata standard name

Geographic information -- Metadata
Metadata standard version

ISO 19115:2003/19139
Metadata author
Organisation name Individual name Electronic mail address Role

Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC)

Institut royal des Sciences naturelles de Belgique (IRSNB), Direction opérationnelle Milieux naturels (DO Nature), Belgian Marine Data Centre (BMDC)

Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN), Operationele Directie Natuurlijk Milieu (OD Natuur), Belgian Marine Data Centre (BMDC)

Königliches Belgisches Institut für Naturwissenschaften (KBIN), Operationelle Direktion Natürliche Umwelt (OD Natur), Belgian Marine Data Centre (BMDC)

bmdc@naturalsciences.be

Ansprechpartner
Point de contact
contactpunt
Point of contact
Other language
Language Character encoding
Deutsch
Allemand
Duits
German
Französisch
Français
Frans
French
Niederländisch
Néerlandais; flamand
Nederlands; Vlaams
Dutch; Flemish
Englisch
Anglais
Engels
English
 
 

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Keywords

GEMET - Concepts, version 4.2.1

marine environment


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