Datum (creatie)

2026-06-16

Datum (revisie)

2026-06-16

Datum (publicatie)

2026-06-18

Datum (creatie)

2016-05-19

Code

https://doi.org/10.24417/bmdc.be:dataset:3144

Identifier

http://metadata.naturalsciences.be / bmdc.be:dataset:3144

Datum

Overige citatie details

This dataset is composed of the following sources: Marta Mikhno, Koen Sabbe (n.d.). Protist community DNA metabarcoding.

Trefwoorden

• Greenland
• nanopore
• fjord
• protist
• Reporting INSPIRE
• Federale Overheid

GEMET - Concepts, version 4.2.3

• DNA

GEMET - Concepts, version 4.2.1

• marine environment

(Juridische) gebruiksbeperking

anders

Overige beperkingen

No conditions apply to access and use

Overige beperkingen

https://creativecommons.org/licenses/by/4.0/

(Juridische) toegangsrestrictie

anders

Overige beperkingen

Geen beperkingen op openbare toegang.

Gebruiksbeperkingen

Geen voorwaarden in verband met gebruik.

Veiligheidsrestricties

vrij toegankelijk

Ruimtelijk schema

tekstTabel

Taal

English

Onderwerp

• oceanen

gml32:beginPosition

2023-07-17

gml32:endPosition

2023-07-31

Referentiesysteem identifier

http://www.opengis.net/def/crs/EPSG/0/4258

Distributie formaat

• application/vnd.ms-excel ( Unknown )

OnLine bronnen

Digital Object Identifier (DOI) ( DOI )

Niveau kwaliteitsbeschrijving

dataset

Algemene beschrijving herkomst

Seawater samples (500 mL) were prefiltered (100 µm) and filtered onto 0.22 µm membrane filters, then stored at −80 °C. DNA was extracted using the DNeasy PowerLyzer Microbial Kit (Qiagen) following the manufacturer’s protocol, including negative extraction controls. The V4 region of the 18S rRNA gene was amplified using the TAReuk454FWD1 and TAReukREV3 primers (Stoeck et al., 2010). Amplicon libraries were sequenced on an Illumina MiSeq platform (2 × 300 bp). Sequence data were processed using the DADA2 pipeline (v.1.14.1) in R. Primers were removed, and reads were quality filtered, trimmed, and truncated (forward reads at 280 bp). Amplicon Sequence Variants (ASVs) were inferred after dereplication and error learning, and chimeras were removed. Due to low quality of reverse reads, only forward reads were retained for downstream analyses. Taxonomic assignment was performed against the PR2 database (v.5.0.0) with a minimum bootstrap confidence of 98%. Subsequent processing (phyloseq) included removal of non-eukaryotic sequences, Metazoa, unassigned ASVs, and potential contaminants (>1% in negative controls).

Metadata ID

bmdc.be:dataset:3144 XML

Taal

English

Karakterset

utf8

Hierarchisch niveau

dataset

Metadata datum

2026-06-18T15:32:47.030Z

Metadata standaard naam.

Geographic information -- Metadata

Metadata standaard versie

ISO 19115:2003/19139