geo.be Metadata Catalog
[UNDER EMBARGO] Bacterial community DNA metabarcoding
UNDER EMBARGO - This dataset is part of BE/2023 sampling campagn in SW Greenland fjords (Igaliku and Tunulliarfik). Samples for DNA extraction were collected along fjord transects at several depths of the water column. The focus of the study was to determine the taxonomical composotion of bacterial community in two Arctic fjords.
Simple
- Date (Creation)
- 2026-06-16
- Date (Revision)
- 2026-10-21
- Date (Publication)
- 2026-10-21
- Title
-
Digital Object Identifier
- Date (Creation)
- 2016-05-19
- Citation identifier
-
http://metadata.naturalsciences.be
/
bmdc.be:dataset:3143
Belgian Marine Data Centre
- Point of contact
-
Organisation name Individual name Electronic mail address Role Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC)
Custodian Royal Belgian Institute of Natural Sciences (RBINS)
Owner Royal Belgian Institute of Natural Sciences (RBINS)
Publisher
- Keywords
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- Greenland
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nanopore
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fjord
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protist
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Reporting INSPIRE
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Federal government
- Use constraints
- Other restrictions
- Other constraints
- No conditions apply to access and use
- Other constraints
- https://creativecommons.org/licenses/by/4.0/
- Access constraints
- Other restrictions
- Other constraints
- No limitations on public access.
- Use limitation
-
No conditions apply to use.
- Classification
- Unclassified
- Spatial representation type
- Text, table
- Language
- English
- Topic category
-
- Oceans
))
- gml32:beginPosition
- 2023-07-17
- gml32:endPosition
- 2023-07-31
- Reference system identifier
- http://www.opengis.net/def/crs/EPSG/0/4258
Digital transfer options
- OnLine resource
-
Protocol Linkage Name DOI
https://doi.org/10.24417/bmdc.be:dataset:3143 Digital Object Identifier (DOI)
- Hierarchy level
- Dataset
Conformance result
- Date (Publication)
- 2010-12-08
- Explanation
-
See the referenced specification
- Pass
- Yes
- Statement
-
Seawater DNA was amplified targeting the full-length 16S rRNA gene using primers 27F and 1492R (Lane, 1991). PCR, library preparation, and barcoding followed Van Der Loos et al. (2021), including mock community and triplicate samples for quality control. Amplicons were sequenced on an Oxford Nanopore MinION platform (R9.4.1 flow cell, 72 h run). Raw reads were basecalled with Guppy, quality-checked (NanoPlot), and demultiplexed (qcat). Chimeras were removed (Yacrd), and reads were filtered by length (1000–2000 bp) and quality (Q >10) using NanoFilt. Sequences were clustered into Operational Taxonomic Units (OTUs) at 97% similarity, and taxonomy was assigned using Kraken2. Downstream processing (phyloseq) included removal of singletons, doubletons, non-bacterial sequences (chloroplasts and mitochondria), unclassified OTUs at phylum level, and potential contaminants (>1% relative abundance in negative controls).
Metadata
- File identifier
- bmdc.be:dataset:3143 XML
- Metadata language
- English
- Character set
- UTF8
- Hierarchy level
- Dataset
- Date stamp
- 2026-10-21T16:13:48.226Z
- Metadata standard name
-
Geographic information -- Metadata
- Metadata standard version
-
ISO 19115:2003/19139
- Metadata author
-
Organisation name Individual name Electronic mail address Role Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC)
Point of contact
- Other language
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Language Character encoding German French Dutch; Flemish English
