bmdc.be:dataset:3143 English UTF-8 Dataset Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC) Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC) Institut royal des Sciences naturelles de Belgique (IRSNB), Direction opérationnelle Milieux naturels (DO Nature), Belgian Marine Data Centre (BMDC) Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN), Operationele Directie Natuurlijk Milieu (OD Natuur), Belgian Marine Data Centre (BMDC) Königliches Belgisches Institut für Naturwissenschaften (KBIN), Operationelle Direktion Natürliche Umwelt (OD Natur), Belgian Marine Data Centre (BMDC) Vautierstraat 29 Vautierstraat 29 Vautierstraat 29 Rue Vautier 29 Vautierstraat 29 Brussel Brussels Brussel Bruxelles Brüssel 1000 Belgium Belgium Belgique België Belgien bmdc@naturalsciences.be https://www.bmdc.be http Point of contact 2026-06-16T13:48:20.317Z Geographic information -- Metadata ISO 19115:2003/19139 http://www.opengis.net/def/crs/EPSG/0/4258 Bacterial community DNA metabarcoding 2026-06-16 Creation 2026-06-16 Revision 2026-06-16 Publication Digital Object Identifier 2016-05-19 https://doi.org/10.24417/bmdc.be:dataset:3143 Belgian Marine Data Centre bmdc.be:dataset:3143 http://metadata.naturalsciences.be This dataset is composed of the following sources: Marta Mikhno, Koen Sabbe (n.d.). Bacterial community DNA metabarcoding. UNDER EMBARGO - This dataset is part of BE/2023 sampling campagn in SW Greenland fjords (Igaliku and Tunulliarfik). Samples for DNA extraction were collected along fjord transects at several depths of the water column. The focus of the study was to determine the taxonomical composotion of bacterial community in two Arctic fjords. Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC) Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC) Institut royal des Sciences naturelles de Belgique (IRSNB), Direction opérationnelle Milieux naturels (DO Nature), Belgian Marine Data Centre (BMDC) Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN), Operationele Directie Natuurlijk Milieu (OD Natuur), Belgian Marine Data Centre (BMDC) Königliches Belgisches Institut für Naturwissenschaften (KBIN), Operationelle Direktion Natürliche Umwelt (OD Natur), Belgian Marine Data Centre (BMDC) Vautierstraat 29 Vautierstraat 29 Vautierstraat 29 Rue Vautier 29 Vautierstraat 29 Brussel Brussels Brussel Bruxelles Brüssel 1000 Belgium Belgium Belgique België Belgien bmdc@naturalsciences.be https://www.bmdc.be http Custodian Laboratorium voor Protistologie en Aquatische Ecologie, Departement Biologie (UGent) Krijgslaan 281 S8, 9000 Gent, Belgium Author Royal Belgian Institute of Natural Sciences (RBINS) Royal Belgian Institute of Natural Sciences (RBINS) Institut royal des Sciences naturelles de Belgique (IRSNB) Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN) Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur) Vautierstraat 29 Vautierstraat 29 Vautierstraat 29 Rue Vautier 29 Vautierstraat 29 Brussel Brussels Brussel Bruxelles Brüssel 1000 Belgium Belgium Belgique België Belgien info@naturalsciences.be https://www.naturalsciences.be http Publisher Royal Belgian Institute of Natural Sciences (RBINS) Royal Belgian Institute of Natural Sciences (RBINS) Institut royal des Sciences naturelles de Belgique (IRSNB) Koninklijk Belgisch Instituut voor Natuurwetenschappen (KBIN) Königliches Belgisches Institut für Naturwissenschaften (KBIN) Natur) Vautierstraat 29 Vautierstraat 29 Vautierstraat 29 Rue Vautier 29 Vautierstraat 29 Brussel Brussels Brussel Bruxelles Brüssel 1000 Belgium Belgium Belgique België Belgien info@naturalsciences.be https://www.naturalsciences.be http Owner Reporting INSPIRE Reporting INSPIRE Reporting INSPIRE Reporting INSPIRE Reporting INSPIRE Federal government Federal government Federale Overheid Gouvernement Fédéral Bundesregierung Other restrictions No conditions apply to access and use https://creativecommons.org/licenses/by/4.0/ Other restrictions No limitations on public access. No limitations on public access. Geen beperkingen op openbare toegang. Pas de restrictions concernant l'accès public. Öffentliche Zugang nicht beschränkt. No conditions apply to use. No conditions apply to use. Geen voorwaarden in verband met gebruik. Aucune condition ne s'applique à l'utilisation. Es gelten keine Bedingungen zum Nutzung. Unclassified Text table English oceans true -46.58078 -45.36212 60.57022 61.15508 2023-07-17 2023-07-31 https://doi.org/10.24417/bmdc.be:dataset:3143 DOI Digital Object Identifier (DOI) Dataset COMMISSION REGULATION (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services COMMISSION REGULATION (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services Verordening (EU) n r. 1089/2010 van de Commissie van 23 november 2010 ter uitvoering van Richtlijn 2007/2/EG van het Europees Parlement en de Raad betreffende de interoperabiliteit van verzamelingen ruimtelijke gegevens en van diensten met betrekking tot ruimtelijke gegevens Règlement (UE) N° 1089/2010 de la Commission du 23 novembre 2010 portant modalités d'application de la directive 2007/2/CE du Parlement européen et du Conseil en ce qui concerne l'interopérabilité des séries et des services de données géographiques Verordnung (EG) Nr. 1089/2010 der Kommission vom 23. November 2010 zur Durchführung der Richtlinie 2007/2/EG des Europäischen Parlaments und des Rates hinsichtlich der Interoperabilität von Geodatensätzen und -diensten 2010-12-08 Publication See the referenced specification See the referenced specification Zie de gerefereerde specificatie Voir la spécification référencée Siehe die angegebene Spezifikation true Seawater DNA was amplified targeting the full-length 16S rRNA gene using primers 27F and 1492R (Lane, 1991). PCR, library preparation, and barcoding followed Van Der Loos et al. (2021), including mock community and triplicate samples for quality control. Amplicons were sequenced on an Oxford Nanopore MinION platform (R9.4.1 flow cell, 72 h run). Raw reads were basecalled with Guppy, quality-checked (NanoPlot), and demultiplexed (qcat). Chimeras were removed (Yacrd), and reads were filtered by length (1000–2000 bp) and quality (Q >10) using NanoFilt. Sequences were clustered into Operational Taxonomic Units (OTUs) at 97% similarity, and taxonomy was assigned using Kraken2. Downstream processing (phyloseq) included removal of singletons, doubletons, non-bacterial sequences (chloroplasts and mitochondria), unclassified OTUs at phylum level, and potential contaminants (>1% relative abundance in negative controls).