geo.be Metadata Catalog
[UNDER EMBARGO] Pelagic community cell abundances and carbon biomass
UNDER EMBARGO - This dataset is part of BE/2023 sampling campagn in SW Greenland fjords (Igaliku and Tunulliarfik). Pelagic community was analysed using Imaging Flow Cytometry (iFCM) with an ImageStream®X Mk II. Cells were grouped into functional size classes—pico-, nano- and microplankton—according to measured cell length. Cells lacking chlorophyll autofluorescence were classified as heterotrophic or chemotrophic organisms, including heterotrophic picoplankton/bacteria (HP; ≤2 µm) and heterotrophic nanoplankton (HN; 2–20 µm). No larger heterotrophs (>20 µm) were visually detected. Autofluorescent cells were considered phototrophic, although this fraction may also include mixotrophic taxa, and comprised picophytoplankton (AP; ≤2 µm), nanophytoplankton (AN; 2–20 µm), and microphytoplankton (AMicro; 20–100 µm). To estimate the biovolume of each plankton class, the two-dimensional cell surface area measured by the IDEAS® imaging software was multiplied by the mean cell width, assuming that cell width approximates the third spatial dimension. Carbon biomass was subsequently derived from biovolume using established carbon–volume relationships. For the HP fraction, carbon content was estimated using the bacterial conversion proposed by Romanova and Sazhin (2010), where volume is expressed in µm³. Although the HP fraction may also include heterotrophic picoeukaryotes, and its biomass may therefore be partly underestimated, this conversion was applied because the fraction was assumed to be numerically dominated by bacteria. For the other protist groups, carbon biomass was derived following Menden-Deuer and Lessard (2000). Carbon values were converted from pg C cell⁻¹ to carbon biomass (µg C L⁻¹) based on cell abundance.
Simple
- Date (Creation)
- 2026-06-16
- Date (Revision)
- 2026-10-21
- Date (Publication)
- 2026-10-21
- Title
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Digital Object Identifier
- Date (Creation)
- 2016-05-19
- Citation identifier
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http://metadata.naturalsciences.be
/
bmdc.be:dataset:3142
Belgian Marine Data Centre
- Point of contact
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Organisation name Individual name Electronic mail address Role Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC)
Custodian Royal Belgian Institute of Natural Sciences (RBINS)
Owner Royal Belgian Institute of Natural Sciences (RBINS)
Publisher
- Keywords
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flow cytometry
- Greenland
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fjord
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Reporting INSPIRE
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Federal government
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- Use constraints
- Other restrictions
- Other constraints
- No conditions apply to access and use
- Other constraints
- https://creativecommons.org/licenses/by/4.0/
- Access constraints
- Other restrictions
- Other constraints
- No limitations on public access.
- Use limitation
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No conditions apply to use.
- Classification
- Unclassified
- Spatial representation type
- Text, table
- Language
- English
- Topic category
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- Oceans
))
- gml32:beginPosition
- 2023-07-17
- gml32:endPosition
- 2023-07-31
- Reference system identifier
- http://www.opengis.net/def/crs/EPSG/0/4258
Digital transfer options
- OnLine resource
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Protocol Linkage Name DOI
https://doi.org/10.24417/bmdc.be:dataset:3142 Digital Object Identifier (DOI)
- Hierarchy level
- Dataset
Conformance result
- Date (Publication)
- 2010-12-08
- Explanation
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See the referenced specification
- Pass
- Yes
- Statement
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Because the upper size detection limit of this instrument (iFCM) is 100 µm, 14 mL of each seawater sample was pre-filtered through a 100 µm mesh to remove larger particles. The filtrate was subsequently fixed with glutaraldehyde (final concentration 1.0%) and stored at 4 °C. Samples were allowed to sediment for at least 48 h, after which the supernatant was carefully removed to obtain a final 7× concentration. This concentration step was necessary to compensate for the relatively low abundance of larger cells within the small analytical volume processed by the instrument.Prior to acquisition, 0.5 mL of each sample was stained with 1% (v/v) SYBR Green I (100× working solution in DMSO) and incubated in the dark at 37 °C for 20 min. Microscopic images (40× magnification; pixel size 0.5 × 0.5 µm) and flow cytometry signals, including brightfield images, fluorescence at 488 nm (SYBR Green–stained nucleic acids), and fluorescence at 642 nm (chlorophyll autofluorescence), were recorded for further analysis.Using IDEAS® software (v. 6.2), plankton populations were identified and quantified based on their optical and fluorescence characteristics.
Metadata
- File identifier
- bmdc.be:dataset:3142 XML
- Metadata language
- English
- Character set
- UTF8
- Hierarchy level
- Dataset
- Date stamp
- 2026-10-21T16:13:47.994Z
- Metadata standard name
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Geographic information -- Metadata
- Metadata standard version
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ISO 19115:2003/19139
- Metadata author
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Organisation name Individual name Electronic mail address Role Royal Belgian Institute of Natural Sciences (RBINS), Directorate Natural Environment (OD Nature), Belgian Marine Data Centre (BMDC)
Point of contact
- Other language
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Language Character encoding German French Dutch; Flemish English
